Faster, more reliable western blots with Stain-Free technology

This guide covers Bio-Rad's Stain-Free technology and how it fits into each step of the western blotting workflow. Readers learn how Stain-Free gels cut total workflow time from 16 hours to 5, let them check transfer before antibody incubation, and support total protein normalization (TPN). TPN is a more accurate alternative to housekeeping proteins and supports today's journal publication guidelines.

Key takeaways:

• Visualize gels minutes after electrophoresis, with no staining or destaining
• Verify protein transfer in as little as 2 minutes
• Use TPN in place of housekeeping proteins for more reliable quantitation