Your step-by-step Stain-Free western blotting protocol

This is a step-by-step bench protocol for running a complete Stain-Free western blot, from sample prep through imaging and analysis. It covers both chemiluminescent and multiplex fluorescent detection, with instrument settings and estimated times for each step. It uses the ChemiDoc Go or ChemiDoc MP Imaging System, the Trans-Blot Turbo Transfer System, and Image Lab Software.