Make your western blot data publication ready with Stain-Free TPN

This poster makes the case for total protein normalization (TPN) as a more reliable standard than housekeeping proteins for western blot quantitation. It compares the two methods side by side, shows how Bio-Rad's Stain-Free workflow completes in about 5 hours versus 10 to 16+ hours for traditional methods, and presents data showing Stain-Free delivers linear quantitation where housekeeping proteins fall short. It also maps how Stain-Free TPN meets normalization requirements from JBC, Nature Portfolio, EMBO Press, and PNAS.

Key takeaways:

• Housekeeping protein expression can vary and signals can saturate, which undermines results
• Stain-Free TPN measures total protein on the same blot, with no extra staining steps
• Meets the normalization requirements of four major journal groups