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How nanopore direct RNA sequencing can enhance your research

Nanopore sequencing is the only technology that lets you directly sequence native RNA without amplification or conversion to cDNA — for the accurate and unbiased analysis you need to discover, develop, and QC the next generation of life-saving drugs.

With Oxford Nanopore sequencing, isoforms can be resolved in full and poly-A tail lengths accurately estimated, and comprehensive quality analysis across the mRNA vaccine development process.

During this webinar, Lin Yang from Oxford Nanopore applications team will introduce how direct RNA sequencing works, from library preparation to data analysis, with workflows and worked examples. Helen Gunter from The University of Queensland will introduce how to use Oxford Nanopore sequencing to trace the lifecycle of an mRNA vaccine, providing quality data from throughout the manufacturing process and insight into its mode of action within the cell.

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Register for free to get video access

What You Will Learn:

  • How the Direct RNA Sequencing Kit enables the preparation and nanopore sequencing of native RNA, including the detection of RNA modifications.
  • About the direct RNA sequencing workflow, including how to prepare libraries with the Direct RNA Sequencing Kit, which sequencing device to choose to meet experimental goals, and how to analyse data.
  • What direct RNA nanopore sequencing can bring to research across numerous applications, with a worked example.
  • Case study of uses Oxford Nanopore sequencing to trace the lifecycle of an mRNA vaccine, providing quality data from throughout the manufacturing process and insight into its mode of action within the cell.

Who Should Attend?

RNA medicines, Cell- and gene-based therapies, drug development, Epigenetics, Multi-attribute testing, single cell, methylation, RNA

Speaker:

helen-gunter-speaker

Helen Gunter

[email protected]
Senior Postdoctoral Fellow,
The University of Queensland

Helen Gunter

Helen Gunter is a Senior Postdoctoral Fellow at the Australian Institute for Bioengineering and Nanotechnology at the University of Queensland. Collaborating with Oxford Nanopore and the BASE mRNA facility, her research aims to develop tools that improve the quality and efficacy of mRNA vaccines. Helen has diverse research and industry experience in genomics, with 17 years in genomics research in Australia and Germany, and as the Project Manager for the second largest genomics facility in the UK.

lin-yang-speaker

Lin Yang

[email protected]
Commercial Applications Manager,
Oxford Nanopore Technologies

Lin Yang

Dr. Lin Yang is Applications Manager at Oxford Nanopore Technologies . Dr Yang graduated from Harvard University with Ph.D. work focusing on long noncoding RNAs in mammalian development. She subsequently moved to the Genome Institute of Singapore for her postdoctoral work in neurodegenerative disease modelling. She has more than 14 years’ experience in cell, molecular and RNA biology. At Oxford Nanopore, she manages the wet lab work of the APAC commercial applications team, working with customers in the region to apply ONT solutions for answering biological questions.