Double Your Plasmid DNA Yield—Without Scaling Up

Scaling plasmid DNA production doesn’t always mean bigger tanks—it starts with smarter bench-scale process design.

In this exclusive Eppendorf Application Note, discover how a side-by-side comparison of batch vs. fed-batch fermentation in the DASbox^® Mini Bioreactor System yielded 56.5 mg of pDNA—a 97% increase over traditional batch (28.7 mg)—using the same E. coli DH5α strain and footprint.

• Exact feed profile & timing that drove the yield boost
• DO-spike as a harvest trigger to avoid plasmid degradation
• Complete media formulations: PAN medium, trace elements, and feed solution
• Real-time process trends: OD, cell wet weight (CWW), and pDNA concentration over 23 hours
• Setup & calibration guidance: headplate configuration, sensor prep, and automation via DASware^® control 6

This isn’t theoretical—it’s a repeatable, data-backed protocol you can implement in your pDNA or viral vector workflow within days.