Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

This article walks researchers through five steps to set up a reliable one-step multiplex RT-qPCR assay, quantifying multiple RNA targets in a single reaction. It covers primer and probe design, reporter selection, validating individual assays, validating the multiplex reaction, and optimizing when needed. It also introduces Reliance One-Step Multiplex RT-qPCR Supermix and PrimePCR Probe Assays.

Key takeaways:

• Get more data from each sample with less pipetting and lower cost
• Clear performance criteria for efficiency and standard curves
• Simple singleplex vs. multiplex check to confirm the assay is ready