MIQE 2.0 Guidelines for Real-Time PCR: Checklist for Authors, Reviewers, and Editors (Bulletin 3556)

MIQE 2.0 updates the minimum reporting standards for qPCR experiments (Bustin et al. 2025). This fillable checklist walks researchers through each requirement across the full workflow, from experimental design and sample handling to reverse transcription, assay validation, and data analysis. It helps labs reduce variability, strengthen reproducibility, and prepare qPCR data for publication.

Key takeaways:

• Covers every stage of the qPCR workflow in one place
• Includes space to record your own lab details for each item
• Built on the 2025 MIQE 2.0 revision